Journal: Frontiers in Molecular Neuroscience

Article Title: Sample Pooling and Inflammation Linked to the False Selection of Biomarkers for Neurodegenerative Diseases in Top–Down Proteomics: A Pilot Study

doi: 10.3389/fnmol.2018.00477

Figure Lengend Snippet: Identification of the candidate biomarker in outliers. (A) Four CJD samples with a 10–15 kDa band of different intensity (arrow). (B) Electrophoresis analysis of the purified candidate biomarker. The arrow marks the position of the peak. (C) After passive elution of the band in (B) , the presence of the candidate biomarker was confirmed by SELDI-TOF analysis with a Q10 ProteinChip Array.

Article Snippet: Each serum sample was diluted 1.5 times with a solution of 8 M urea, 1% CHAPS and stirred at room temperature for 15 min. ProteinChip Q10 Arrays (anion exchanger) (Bio-Rad) were pre-equilibrated with 150 μl of binding buffer (100 mM Tris pH 9 (made from TrisBase adjusted using HCl solution) and 0.1% Triton X-100) in a 96-well bioprocessor with gentle agitation for 5 min. Then, 2 μl of denaturated sample was mixed with 100 μl of binding buffer.

Techniques: Biomarker Discovery, Electrophoresis, Purification